Dupilumab attenuates the expression of TSLP and IL-8 induced by dsRNA and IL-4/IL-13 co-stimulation in human small airway epithelial cells

by Aditya Sri Listyoko, Ryota Okazaki, Tomoya Harada, Genki Inui, Hiroki Kohno, Miyu Nishigami, Miki Takata, Masato Morita, Akira Yamasaki

Background

The interaction between viral components and type 1 or type 2 cytokines during asthma exacerbations in the airway epithelium may contribute to worsening inflammation. However, these interactions in the small airway epithelium—particularly those involving alarmins (TSLP, IL-25, and IL-33) and IL-8—remain unclear. Dupilumab, a biologic agent used in severe asthma, blocks IL-4 receptor alpha (IL-4Rα) and may offer therapeutic benefits in virus-induced asthma exacerbations. In this study, we evaluate the effects of double-stranded RNA (dsRNA), in combination with various cytokines and dupilumab, on the Human Small Airway Epithelial Cells (HSAECs) line.

Methods

Primary HSAECs were preincubated with dsRNA to induce the gene and protein expression of alarmins and IL-8. To evaluate the effects of cytokines on dsRNA-induced alarmin and IL-8 expression, various type 1 and type 2 cytokines were co-stimulated with dsRNA. Dupilumab was used as a pretreatment prior to co-stimulation with dsRNA and IL-4 or IL-13. Gene expression of TSLP, IL-25, IL-33, and IL-8 was assessed by quantitative PCR, and protein expression was evaluated by Western Blotting.

Results

dsRNA significantly increased the expression of TSLP and IL-8. IL-4 and IL-13 further enhanced dsRNA-induced TSLP and IL-8 gene and protein expression. In contrast, TNF-α reduced dsRNA-induced TSLP expression but enhanced IL-8 gene and protein expression. Dupilumab attenuated the expression of TSLP and IL-8 induced by co-stimulation with dsRNA and IL-4 or IL-13 in HSAECs.

Conclusion

In the microenvironment of small airway epithelial cells, particularly during viral infections, the presence of IL-4 or IL-13 may enhance the expression of TSLP and IL-8. Dupilumab attenuates this expression, potentially offering additional benefits in the treatment of asthma, especially during virus-induced asthma exacerbations.

Dominance of <i>Zygosaccharomyces</i> and shifts in bacterial pathways: Effects of antimicrobials on composition and diversity of the <i>Ceratitis capitata</i> bacterial and fungal microbiome

by Maria Cecilia Rasuk, Alfonsina Palladini, Andrea Moyano, Viviana Díaz, Antonella Giudice, Gisela Castillo, Solana Abraham, Juan Rull, Anja Poehlein, Rolf Daniel, Julian Rafael Dib

The Mediterranean fruit fly (Ceratitis capitata Wied.) is an agricultural pest of significant economic importance. This species has been globally managed using the Sterile Insect Technique (SIT). Insects, including tephritid flies, harbor a diverse gut microbiota that plays critical roles in their physiology, behavior, and overall fitness, suggesting that microbial communities may profoundly influence the biology of this pest. The aim of this study was to characterize the fungal and bacterial gut microbial communities of C. capitata from Tucumán, Argentina, and to assess their response to antimicrobial treatment using amplicon-based 16S rRNA gene and ITS region sequencing. Both control and treated flies were dominated by Proteobacteria (bacteria) and Zygosaccharomyces (fungi). Antimicrobial treatment induced significant shifts in bacterial and fungal composition, reducing diversity and altering gut community structure. Untreated flies exhibited a diverse and structured bacterial gut community dominated by the family Enterobacteriaceae, while antibiotic-treated communities were dominated by Rhizobiaceae. Despite these shifts, fungal communities in both treated and untreated groups were consistently dominated by the genus Zygosaccharomyces. Functional predictions revealed notable changes in metabolic pathways following antibiotic treatment, including increased gene abundance for ABC transporters and the phosphotransferase system, and decreased representation of genes involved in antibiotic biosynthesis and two-component systems. These results indicate significant alterations in bacterial metabolism and stress response mechanisms induced by the treatment. Such changes may help explain the underperformance of irradiated, mass-reared males within the context of SIT. This study provides new insights into the structural and functional dynamics of the C. capitata gut microbiome under disturbance. These findings have implications for understanding the ecological roles of microbial communities in this pest and their potential impact on fly health and fitness. Identification of dominant gut bacterial and fungal groups may support the development of probiotic diets, enhancing the efficiency of SIT application.

Photobiomodulation for postoperative pain relief following conventional periapical surgery: a randomised controlled study protocol

Introduction

Photobiomodulation (PBM) has shown promising effects in managing postoperative pain following conventional periapical surgery, although current evidence remains limited. This study aims to assess the effect of PBM on postoperative pain 24 hours after periapical surgery.

Methods and analysis

A randomised, controlled, double-blind trial will include 34 patients undergoing periapical surgery in the maxillary region, randomly assigned to an experimental group (n=17) or control group (n=17). The experimental group will receive PBM (GaAlAs diode laser, 808 nm, 100 mW, 4 J/cm², applied at five vestibular points) and placebo ibuprofen immediately and 24 hours postoperatively. The control group will receive simulated PBM and active ibuprofen. The primary outcome is postoperative pain assessed by the visual analogue scale at 24 hours. Secondary outcomes include pain at the seventh day, paracetamol intake, oedema, ecchymosis, soft tissue status and temperature at 24 hours and 7 days. Radiographic evaluation of healing will be performed at 1 and 3 months. Statistical analysis will be conducted based on data distribution, using repeated measures ANOVA (Analysis of Variance) or non-parametric equivalents for longitudinal outcomes, and appropriate tests for categorical variables. Significance will be set at p

Ethics and dissemination

The study was approved by the Human Research Ethics Committee of Universidad Católica del Uruguay (process no. 220914). Results will be disseminated to participants, healthcare professionals, the public and scientific communities.

Trial registration number

NCT05935306.

Effectiveness of an Online Training Program on Brief Tobacco Intervention (BTI) for Nurses: A Quasi‐Experimental Study. The E‐Learning BTI Project

ABSTRACT

Introduction

Smoking is the leading cause of preventable deaths. The training of professionals on brief tobacco interventions (BTIs) increases the effectiveness of these interventions.

Objective

To assess the effectiveness of an online training program on BTI based on the 5As and 5Rs model in acquiring anti-tobacco brief advice competencies among nurses.

Method

Quasi-experimental study with a pre-test and post-test design, with a control group and without random assignment. In the experimental group (EG), online training was provided in three sections: BTI theoretical content and methodology, clinical scenario videos, and feedback. Each scenario assessed the 5As and 5Rs as a validated instrument (BTI-Prof^(C)). The control group (CG) only assessed the three videos of clinical scenarios. In both groups, competence was measured at the following points in time: T0 (before the training), T1 (at the end of the training), and T2 (after 90 days). The efficacy of the intervention was measured through a two-way ANOVA, and the variation rate was calculated from T0 to T1 and from T0 to T2.

Results

236 nurses participated (157 EG; 79 CG). The mean age was 42.9 years, and 76.7% were women. There was a significant group*time interaction in the three cases, indicating that the online BTI training increases the competence of these professionals in clinical scenario 1 (F = 10.210; p ≤ 0.001; η ² = 0.081), clinical scenario 2 (F = 6.235; p = 0.002; η ² = 0.051), and clinical scenario 3 (F = 11.271; p ≤ 0.001; η ² = 0.090).

Conclusion

A brief, asynchronous, and online intervention using standardized video-based cases is effective in improving nurses' BTI competence. This type of training can be a useful option for the National Health System as part of a global and continuous strategy for nurses to perform BTI.

Clinical Relevance

An asynchronous online training program provides nurses with standardized, evidence-based tools to implement brief tobacco interventions in routine care, offering a scalable and practical solution to strengthen preventive strategies in health systems.

Relación entre autocuidado y automedicación en estudiantes de ciencias de salud de una universidad pública

Objetivo. Analizar la prevalencia de la automedicación y su relación con la capacidad de autocuidado en estudiantes del Centro de Ciencias de la Salud de una universidad pública. Metodología. Se llevó a cabo un estudio cuantitativo, relacional y observacional con una muestra de 330 estudiantes seleccionados aleatoriamente. Se utilizaron dos instrumentos validados para medir la automedicación y la capacidad de autocuidado, además de un formulario para variables sociodemográficas. Los datos se analizaron con SPSS versión 25, utilizando pruebas de normalidad, independencia y Chi-cuadrado de Pearson. Resultados. El 81,5% de los estudiantes presentó una capacidad media de autocuidado, mientras que el 18,5% tuvo una capacidad alta. No se encontraron estudiantes con baja capacidad de autocuidado. La prevalencia de la automedicación fue del 73,9%, siendo los fármacos más comunes antidiarreicos (10,6%), anfetaminas (10%), anticonceptivos orales y acetaminofén (7,6% cada uno). Los síntomas principales para automedicarse fueron dolor estomacal (9,7%), desgano/agotamiento (9,4%) y dolor de cabeza (9,1%). La carrera de Enfermería mostró la mayor prevalencia de automedicación (80,6%), seguida de Terapia Física (78,6%) y Medicina (76,4%). Se identificó dependencia estadísticamente significativa entre automedicación y capacidad de autocuidado. Discusión. Existe alta prevalencia de automedicación entre los estudiantes del área de salud. Es crucial reevaluar la independencia de ambas variables y abordar los factores que llevan a los estudiantes a automedicarse, ya que este comportamiento podría afectar su capacidad para proporcionar cuidados seguros y adecuados a los pacientes. Aun cuando no hay independencia entre variables.

ABSTRACT

Objective. To analyze the prevalence of self-medication and its relationship with the capacity of self-care among students of the Health Sciences Center in a public university. Methodology. A quantitative, relational and observational study was performed to a sample group of 330 students randomly selected. Two validated instruments were used to measure self-medication and self-care capacity, as well as a form for sociodemographic variables. All data were analyzed utilizing SPSS version 25, and using tests of normality, independence, and Pearson's Chi-square. Results. 81.5% of the students showed having a medium capacity of self-care, while 18.5% showed having a high capacity. Students with low self-care capacity were not found. The prevalence of self-medication was 73.9%, with the most common drugs being antidiarrheals (10.6%), amphetamines (10%), oral contraceptives, and acetaminophen (7.6% each). The main symptoms for self-medication resulted being stomach pain (9.7%), apathy / exhaustion (9.4%), and headache (9.1%). The Nursing career resulted with the highest prevalence of self-medication (80.6%), followed by Physical Therapy (78.6%) and Medicine (76.4%). A statistically significant dependency was identified between self-medication and self-care capacity. Discussion. There is a high prevalence of self-medication among health students. It is crucial to reevaluate the independence of both variables and address the factors that lead students to self-medication, as this behavior could affect their ability to provide safe and appropriate care to patients. Even when there is no independence between variables.

Representación de la enfermedad en personas adultas costarricenses con hipertensión arterial

Objetivo: Describir la representación de la enfermedad que tienen las personas adultas costarricenses con hipertensión arterial. Metodología: Estudio descriptivo, con muestreo por conveniencia de 81 personas adultas con hipertensión arterial. La representación de la enfermedad se midió con el Cuestionario Breve de Percepción de la Enfermedad. Los datos se analizaron con estadística descriptiva. Resultados principales: Se evidenció una representación de la enfermedad poco amenazante. La dimensión duración presentó mayor grado de amenaza y la emocional, control personal y del tratamiento, y coherencia menor grado de amenaza. Las principales causas de hipertensión arterial percibidas fueron estrés, genética y nutrición. Conclusión principal: Las personas perciben la hipertensión arterial poco amenazante, lo cual podría llevarlos a tener dificultades para adoptar conductas saludables. Una mejor comprensión de las representaciones de las enfermedades en la población costarricense tiene el potencial de dirigir futuras intervenciones para mejorar el bienestar.

Consecuencias de las caídas en un centro geriátrico para ancianos válidos. ESTUDIO DESCRIPTIVO.

Rev Enferm;39(6): 50-4, 2016 Jun. . [Artículo]