Conectar
by Ying Fei, Ming-Yi Gao, Nan Qiao, Jia Hu, Ling He, Jiao-Li Zhou, Ning-Ning Zheng, Ting-Ting Liu
BackgroundThe effect of fecal microbiota transplantation (FMT) in treating irritable bowel syndrome (IBS) may be attributed to the modulation of CD8 + T cells. This study aims to identify FMT-mediated key genes to explore the underlying mechanism.
MethodsTranscriptomic datasets GSE138297 (colonic biopsies from 8 IBS patients pre- and post-FMT) and GSE134649 (single-cell data from 3 healthy colon tissues) were obtained from GEO during December 2023–December 2024. Key genes were identified by intersecting differentially expressed genes (DEGs) and the most relevant co-expression module derived from weighted correlation network analysis. Functional enrichment, gene set enrichment analysis, immune infiltration profiling via TIMER 2.0, single-cell annotation using PanglaoDB and Seurat, and drug–gene interaction screening from DrugBank were conducted to decipher the regulatory mechanisms.
ResultsTen key genes were identified through integration of DEGs and the MEgreen module. Functional analyses revealed significant involvement in the positive regulation of CD8 + T cells activation. Immune infiltration assessment demonstrated a marked increase in CD8 + T cells abundance post-FMT. Single-cell data indicated predominant expression of LILRB1, P2RY13, CLEC10A, and CLEC12A in dendritic cells, and LILRB1, PIPOX, and CLEC11A were annotated within CD8 + T cells clusters in healthy colonic tissue. Nine (database-derived and speculative) drugs targeting seven key genes were identified, most implicated in the management of IBS symptoms or immunomodulation.
ConclusionAn association between key gene regulation and CD8 + T cell-related immunoregulation is correlated with the therapeutic effect of FMT in IBS.
To conduct a comprehensive assessment of nursing interventions for patients with hypertension, diabetes, and dyslipidemia and analyse the components, delivery methods and outcomes of intervention programmes.
Scoping review.
Systematic searches were performed in four Chinese databases (WanFang, CNKI, Chinese Biomedical Literature Database, and the VIP database) and six English databases (CINAHL, MEDLINE, Web of Science, PubMed, Embase, The Cochrane Library) from their inception until October 2023. An updated search was performed on 6 August 2024.
Two reviewers independently retrieved full-text studies and conducted the initial screening of titles and abstracts, followed by full-text analysis and data extraction.
A total of 49 articles were included in this review. The nursing interventions consisted of various components, including fitness exercise, a balanced diet, mental health support, medication administration and others. The most commonly used delivery method was health education, with an increasing trend towards online interventions. However, the included studies did not provide details on delivery methods, including the team qualifications, subject areas or intervention duration and frequency. The nursing interventions achieved their research aims to varying degrees, as measured by subjective and/or objective indicators.
The nursing interventions for the three highs are diverse, including offline, online and combined methods, covering exercise, diet, and mental health. Future efforts can draw on these intervention components and methods and establish a nurse-led multidisciplinary team. The measurement of objective indicators, including blood lipids, should be taken seriously. Developing more diverse subjective measurement indicators can comprehensively assess patients' health.
This review offers clear guidance for the subsequent prevention and management of the three highs and consolidates evidence for healthcare professionals to devise targeted intervention strategies.
We followed Arksey's five-step framework and the PRISMA extension for scoping reviews (PRISMA-ScR).
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FreshRSS 