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by Juan F. Zapata-Acevedo, Mónica Losada-Barragán, Johann F. Osma, Juan C. Cruz, Andreas Reiber, Klaus G. Petry, Amael Caillard, Audrey Sauldubois, Daniel Llamosa Pérez, Aníbal José Morillo Zárate, Sonia Bermúdez Muñoz, Agustín Daza Moreno, Rafaela V. Silva, Carmen Infante-Duarte, William Chamorro-Coral, Rodrigo E. González-Reyes, Karina Vargas-Sánchez
Chronic neuroinflammation is characterized by increased blood-brain barrier (BBB) permeability, leading to molecular changes in the central nervous system that can be explored with biomarkers of active neuroinflammatory processes. Magnetic resonance imaging (MRI) has contributed to detecting lesions and permeability of the BBB. Ultra-small superparamagnetic particles of iron oxide (USPIO) are used as contrast agents to improve MRI observations. Therefore, we validate the interaction of peptide-88 with laminin, vectorized on USPIO, to explore BBB molecular alterations occurring during neuroinflammation as a potential tool for use in MRI. The specific labeling of NPS-P88 was verified in endothelial cells (hCMEC/D3) and astrocytes (T98G) under inflammation induced by interleukin 1β (IL-1β) for 3 and 24 hours. IL-1β for 3 hours in hCMEC/D3 cells increased their co-localization with NPS-P88, compared with controls. At 24 hours, no significant differences were observed between groups. In T98G cells, NPS-P88 showed similar nonspecific labeling among treatments. These results indicate that NPS-P88 has a higher affinity towards brain endothelial cells than astrocytes under inflammation. This affinity decreases over time with reduced laminin expression. In vivo results suggest that following a 30-minute post-injection, there is an increased presence of NPS-P88 in the blood and brain, diminishing over time. Lastly, EAE animals displayed a significant accumulation of NPS-P88 in MRI, primarily in the cortex, attributed to inflammation and disruption of the BBB. Altogether, these results revealed NPS-P88 as a biomarker to evaluate changes in the BBB due to neuroinflammation by MRI in biological models targeting laminin.by Marcos Antonio Matiucci, Iza Catarini dos Santos, Natallya Marques da Silva, Patricia Daniele Silva dos Santos, Gislaine Gonçalves Oliveira, Stefane Santos Corrêa, Elder dos Santos Araujo, Rafaela Said, Jaqueline Ferreira Silva, Ana Paula Sartório Chambó, Talita Aparecida Ferreira de Campos, Oscar Oliveira Santos, Claudete Regina Alcalde, Maria Luiza Rodrigues de Souza, Andresa Carla Feihrmann
The effect of the use of Nilo tilapia filleting residues in the production of pâtés with the addition of oregano essential oil stored for 90 days at 4 °C was evaluated. For that, 5 treatments were performed as follows: TSA—control treatment; TES with the addition of sodium erythorbate; and formulation TOE1 with 600 ppm oregano essential oil; TOE2 with 1000 ppm essential oil; and TOE3 with 1400 ppm essential oil. The pâtés showed adequate technological and physicochemical characteristics and microbiological counts within the legislation standards. No significant differences were observed in the luminosity of the pâté formulations during storage, and the addition of oil contributed to the increase in a* values and stability of b* values. Regarding the lipid and protein oxidation, TOE3 showed lower values at the end of the shelf-life. The addition of essential oil did not affect the hardness and cohesiveness of the products. The fatty acids in greater amounts in the samples were linoleic, oleic, palmitic, and stearic acids. The analysis of biogenic amines indicated that only the treatments with the highest amounts of sodium erythorbate (TES and TOE1) showed losses of spermidine. It was observed that decreasing the inclusion of sodium erythorbate and increasing the inclusion of oregano essential oil resulted in a drop in cadaverine values. A total of 46 volatile compounds were detected in the samples with the highest amount of free fatty acids and all the formulations were well accepted sensorially.Las TICs permiten que el estudiante acceda al material de estudio y, a su vez, interactúe con el profesor y con otros estudiantes. La pandemia COVID-19 ha obligado a docentes a impartir clases virtuales de urgencia para cumplir con las actividades académicas programadas.
El objetivo es analizar el nivel de satisfacción de docentes en el desarrollo de las clases virtual en tiempos de pandemia. El diseño fue observacional, descriptiva, de corte transversal, enfoque cuantitativo, el universo estuvo compuesto por docentes de la FENOB-UNA Casa Central y Filiales, con una muestra de 114 docentes, la selección se realizó a través de un muestreo no probabilístico de casos consecutivos, la recolección de datos se hizo a través de una encuesta en línea con un cuestionario elaborado por las investigadoras, validada por expertos. Los hallazgos más relevantes fueron: mayoritariamente de sexo femenino, del grupo etario de 36 a 55 años, de estado civil casado, procedentes del departamento Central, son docente catedráticos y ejercen su labor solamente en la FENOB-UNA, cuenta con conexión a internet, utiliza computadora portátil y destina 2 horas para las clases. El análisis entre las características sociodemográficas y el nivel de satisfacción con el desarrollo de las clases virtuales es estadísticamente significativo con la variable edad. Se concluye que el nivel de satisfacción de docentes en el desarrollo de las clases virtuales en tiempos de pandemia fue medianamente satisfecho.
ABSTRACT
ICTs allow the student to access the study material and, in turn, interact with the teacher and other students. The COVID-19 Pandemic has forced teachers to teach emergency virtual classes to comply with scheduled academic activities. The objective is to analyze the level of satisfaction of teachers in the development of virtual classes in times of pandemic. The design was observational, descriptive, cross-sectional, quantitative approach, the universe was made up of teachers from the FENOB- UNA Central House and Subsidiaries, with a sample of 114 teachers, the selection was made through a non-probabilistic sampling of cases. consecutive, data collection was done through an online survey with a questionnaire prepared by the researchers, validated by experts. The most relevant findings were: mostly female, in the age group of 36 to 55 years, with married marital status, from the Central Department, are professors and work only at FENOB-UNA, have an internet connection, He uses a laptop and allocates 2 hours for classes. The analysis between the sociodemographic characteristics and the level of satisfaction with the development of virtual classes is statistically significant with the age variable. It is concluded that the level of satisfaction of teachers in the development of virtual classes in times of pandemic was moderately satisfied.
Objetive: analizar las percepciones, motivaciones y dificultades de los técnicos de enfermería titulados en enfermería. Metodología: Me-todología: Se trata de una investigación cualitativa, exploratoria descriptiva, la recolección de datos se realizó en un Hospital Municipal del Noroeste de Paraná, durante el primer semestre de 2020, mediante la aplicación de un guión semiestructurado con preguntas orien-tadoras y un cuestionario sociodemográfico. Resultados: El conocimiento adquirido en la graduación de enfermería fue uno de los facto-res facilitadores que contribuyó a una mejor ejecución de la atención brindada, y la mayor dificultad está relacionada con la aceptación de los colegas y supervisores, en relación a las opiniones y sugerencias sobre los procedimientos u organización de la Servicio. Las razones que llevaron a los técnicos de enfermería a cursar una carrera en el área en la que trabajan se deben a la necesidad de cono-cimientos y la búsqueda de un mejor futuro financiero. Conclusión: cuando el profesional tiene un título, se desarrolla con mayor destreza y eficacia en la asistencia proporcionada.
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