Isolation and characterization of a <i>Chlamydia muridarum tc0237</i> mutant from a genetic screen that is attenuated in epithelial cells

by Kaylee R. Jacobs, Caleb M. Ardizzone, Arkaprabha Banerjee, Evelyn Toh, Xiaoli Zhang, David E. Nelson

Chlamydia are obligate intracellular bacterial pathogens that infect a wide range of vertebrate hosts. Despite having highly conserved genomes, closely related Chlamydia species can exhibit distinct host and tissue tropisms. The host tropisms of the human pathogen Chlamydia trachomatis and the closely related mouse pathogen Chlamydia muridarum are influenced by their ability to evade host immune responses, particularly those mediated by interferon gamma. However, there is evidence that tissue tropism is driven by additional poorly understood host and Chlamydia factors. In this study, we used a forward genetic approach to investigate the mechanisms that mediate C. muridarum tissue tropism. We conducted a tropism screen using a randomly mutagenized C. muridarum library and murine cell lines representing different tissues. We identified a mutant isolate whose growth was restricted in murine rectal and oviduct epithelial cells in an interferon gamma-independent manner. This phenotype was mapped to a missense mutation in tc0237, a gene that mediates the affinity of C. muridarum for cultured human epithelial cells. Our analysis of growth dynamics showed that the tc0237 mutant exhibits a developmental delay in rectal epithelial cells. Together, these results suggest that TC0237 plays a role in C. muridarum tissue tropism.

Effectiveness of Massage Therapy for Cancer Pain, Quality of Life and Anxiety Levels: A Systematic Review and Meta‐Analysis

ABSTRACT

Aim

To synthesise the effectiveness of massage therapy for cancer pain, quality of life and anxiety among patients with cancer.

Design

Systematic review and meta-analysis.

Methods

This review was reported according to the PRISMA guidelines. Studies evaluating the effects of massage therapy on cancer pain, quality of life or anxiety in patients with cancer pain were eligible. The Cochrane Risk of Bias tool and Grading of Recommendations Assessment, Development and Evaluation were used to assess the quality of studies. Outcomes were pooled using standardised mean differences and narratively synthesised when meta-analysis was not possible.

Data Sources

Pubmed, EMBASE, Web of Science, CINAHL, CENTRAL, Google Scholar, ProQuest Theses and Dissertations were searched for English peer-reviewed studies and grey literature published from inception to 8 January 2024.

Results

Thirty-six RCTs involving 3671 participants were included. Massage therapy significantly improved pain (pooled SMD = −0.51, 95% CI −0.68 to −0.33), quality of life (pooled SMD = 0.48, 95% CI 0.19–0.78 when higher scores indicate better quality of life; pooled SMD = −0.52, 95% CI −0.88 to −0.16 when higher scores indicate poorer quality of life) and anxiety (pooled SMD = −0.38, 95% CI: −0.57 to −0.18) post-intervention. All outcomes had very low certainty of evidence. Most studies had unclear or high risk of bias.

Conclusion

This review found that massage therapy is beneficial to patients with cancer in improving pain, quality of life and anxiety. Healthcare institutions and healthcare professionals should recognise the value of massage therapy to enhance the care of patients with cancer pain.

Reporting Method

PRISMA guidelines.

Patient or Public Contribution

No Patient or Public Contribution.

Registration

PROSPERO CRD42023407311.