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To further elucidate the effects of rare systemic autoimmune rheumatic diseases (SARD) and their treatment on antibody development after vaccination against SARS-CoV-2, we compared patients with and without immunosuppressive therapy to healthy controls in an observational cohort study.
We enrolled 52 patients with SARD and 72 healthy subjects in a prospective, observational study at the Medical University of Vienna and measured the humoral response 6 months after two mRNA vaccinations and 2–6 weeks after a third dose.
Patients with vasculitis showed significantly (p=0.02) lower antibody titres 6 months after vaccination (median 247 BAU/mL, IQR [185–437]), as compared with healthy controls (median 514 BAU/mL, [185–437], IQR 323; 928, vasculitis patients: 247, IQR [185; 437], p
Patients with SARD displayed lower antibody development after booster vaccination, even if antibody levels after two immunisations were comparable to healthy controls. Our data may be limited due to sample size, but it provides pointers for a more individualised, antibody-titre-oriented approach and earlier booster vaccination in patients with SARD.
by Mai Dinh Thanh, Gemma Agustí, Anneluise Mader, Francesc Codony
Staphylococcus (S.) aureus is a prominent foodborne pathogen that can cause food poisoning due to its staphylococcal toxins. Controlling the viable levels of S. aureus is crucial for ensuring food safety. The detection of S. aureus during routine quality control is still primarily conducted using traditional culture-based methods, which are time-consuming and unable to detect viable but non-culturable cells. Viability PCR (vPCR) – a combination of traditional (or quantitative) PCR with photo-reactive DNA-intercalating dye(s) – has been introduced as an alternative to detect viable cells by excluding those with compromised membranes using molecular methods. Despite the success of the vPCR methodology, avoiding false-positive results remains a significant challenge. To enhance the accuracy of vPCR results for S. aureus, several approaches have been proposed by various researchers in the past decade; however, complete PCR signal suppression of dead cells has not been achieved. In this study, we developed a strategy to detect only viable S. aureus cells by combining double PMA treatment with a low PMA concentration and performing a tube change between the last dark incubation and light exposure to improve the vPCR protocol. For pure cultures, the optimized protocol was able to completely suppress DNA signals from 5.0 × 107 dead cells in a final reaction volume of 200 µl. For artificially contaminated food samples with such a high dead cell count, complete PCR signal reduction was observed in ground pepper, - oregano, and infant milk powder, while ground paprika, - allspice, and - pork exhibited PCR signals close to the detection limit. To simulate conditions in real samples, we artificially contaminated ground paprika, - pork, and milk powder with a low number of viable cells (~1.9 cfu/ml) and a high number of heat-inactivated S. aureus (~4.8 × 10⁶ cells/ml). The results showed that the optimized protocol is effective in detecting only the desired live cells, even in the presence of a high dead cell count. Our findings highlight that vPCR can be an accurate and reliable method with strong potential for high-throughput detection of live S. aureus cells in certain food matrices.This study assessed the health-related quality of life (HRQoL) and its relationship with clinical factors and comorbidities in people with type 2 diabetes mellitus (T2DM) treated in primary care settings.
Cross-sectional study design: This study assessed the HRQoL using a 36-item Short Form Survey (SF-36) tool in eight domains. The HRQoL scores ranged from 0% to 100% for each domain, with higher scores indicating better HRQoL. Linear regression was used to assess the association of HRQoL domain scores with clinical covariates and comorbidities.
A countrywide study was conducted on individuals with established T2DM (N=635) attending primary healthcare services for various conditions across nine federal states of Austria from 2021 to 2023.
A total of 635 individuals, aged above 50 years and diagnosed with T2DM, were recruited by the attending physician to evaluate their HRQoL in relation to T2DM and its associated comorbidities.
The mean SF-36 scores for physical functioning (69±28), role-physical (62±42), mental health (72±20), role-emotional (73±41), social functioning (79±25), bodily pain (67±28) and vitality (55±22) were satisfactory, except for general health (41±10). Age and body mass were inversely associated with physical, mental and social HRQoL (p
Our analysis showed that advanced age, obesity, depression, cardiovascular disease, chronic lung diseases and multimorbidity were associated with poor HRQoL of individuals with T2DM at the primary care level. These findings highlight the need for strengthening holistic management in primary care to address the diverse physical, social and emotional needs of individuals with T2DM.
Objetivo: Demostrar mediante la monitorización con el índice biespectral la reducción de complicaciones asociadas a sobresedación en paciente con ventilación mecánica. Metodología: Enfermería basada en evidencia. Traducción a lenguaje científico DeCS y MeSH. Fuentes documentales: Epistemonikos, PUBMED, ELSEVIER, DOAJ y Google académico. Operador booleano AND, OR y NOT. Lectura critica mediante FLC 3.0. Jerarquización con Scottish Intercollegiate Guidelines Network (SIGN). Resultados: Ocho de 10 artículos demuestran reducción de eventos adversos, 5 de 10 artículos indican reducción de dosis empleadas y administradas de sedantes, también una fuerte evidencia de la precisión para valorar el nivel de sedación y reducción de la incidencia de la sedación. Se necesitan más estudios. Conclusión: La monitorización con índice Biespectral tiene un índice de especificidad más objetivo que escalas de sedación comúnmente usadas y demostró que reduce complicaciones asociadas a la sobresedación. Se requieren más estudios.
Objetivo principal: Analizar la evidencia científica sobre la fototerapia convencional vs la fototerapia con terapia de masaje para la disminución de los niveles de bilirrubina en neonatos a término (37 a 42 SDG) con hiperbilirrubinemia > 5 mg/dL en la terapia intensiva neonatal. Metodología: Enfermería Basada en Evidencia. Bases de datos consultadas: Google académico, PubMed. Elaboración de tablas con los Descriptores en Ciencias de la Salud (DeCS). La lectura crítica se realizó usando CASPe y el nivel de evidencia de acuerdo al Centre for Evidence-Based Medicine, Oxford. Resultados principales: Doce artículos describen que la terapia de masaje aumenta la actividad vagal, dando como resultado una mayor motilidad gástrica y una disminución más rápida de los niveles de bilirrubina. Conclusión principal: la terapia de masaje es una intervención complementaria efectiva durante la fototerapia para reducir los niveles de bilirrubina neonatal.
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