Developing policy recommendations for controlling energy drink consumption in secondary school students using social marketing theory, Shiraz, Iran: A study protocol

by Mohammadhassan Rostami, Mina Babashahi, Masoud Karimi, Soheila Khodakarim

Background

Energy drink (ED) consumption has risen sharply among children and adolescents, posing health risks such as obesity and overweight, heart problems, mood disorders, and insomnia. Recognizing these concerns, international organizations have issued guidelines discouraging adolescent ED consumption, leading to policy measures in many countries. This study leverages social marketing theory to develop targeted policy recommendations for managing ED consumption in secondary school students.

Methods

This study utilizes a cross-sectional design with a mixed-methods approach to collect data and formulate policy recommendations. A multistage cluster sampling method was employed to randomly select students from 24 schools, serving as the primary data source. Information is gathered through a questionnaire based on the Theory of Planned Behavior (TPB). Additionally, a food environment analysis of the selected schools, a critical factor influencing ED consumption, is conducted using the NEMS-S INFORMAS tool. This tool assesses the availability, pricing, and marketing of EDs. The study further explores stakeholder perspectives through key informant interviews and a systematic literature review, providing valuable insights into existing policy frameworks. The study aims to develop actionable policy recommendations to effectively address ED consumption by synthesizing findings from all these phases.

Discussion

The social marketing model focuses on understanding the audience and evaluating outcomes to develop effective policy proposals. It is particularly useful for behavior change policies, offering evidence-based recommendations that often surpass traditional health promotion methods. This study will analyze ED consumption and its influencing factors using the model’s constructs to present informed and practical policy recommendations.

<i>In vivo</i> monitoring of leukemia-niche interactions in a zebrafish xenograft model

by Anja Arner, Andreas Ettinger, Bradley Wayne Blaser, Bettina Schmid, Irmela Jeremias, Nadia Rostam, Vera Binder-Blaser

Acute lymphoblastic leukemia (ALL) is the most common type of malignancy in children. ALL prognosis after initial diagnosis is generally good; however, patients suffering from relapse have a poor outcome. The tumor microenvironment is recognized as an important contributor to relapse, yet the cell-cell interactions involved are complex and difficult to study in traditional experimental models. In the present study, we established an innovative larval zebrafish xenotransplantation model, that allows the analysis of leukemic cells (LCs) within an orthotopic niche using time-lapse microscopic and flow cytometric approaches. LCs homed, engrafted and proliferated within the hematopoietic niche at the time of transplant, the caudal hematopoietic tissue (CHT). A specific dissemination pattern of LCs within the CHT was recorded, as they extravasated over time and formed clusters close to the dorsal aorta. Interactions of LCs with macrophages and endothelial cells could be quantitatively characterized. This zebrafish model will allow the quantitative analysis of LCs in a functional and complex microenvironment, to study mechanisms of niche mediated leukemogenesis, leukemia maintenance and relapse development.

Multiparameter immunoprofiling for the diagnosis and differentiation of progressive versus nonprogressive nontuberculous mycobacterial lung disease–A pilot study

by Paige K. Marty, Balaji Pathakumari, Thomas M. Cox, Virginia P. Van Keulen, Courtney L. Erskine, Maleeha Shah, Mounika Vadiyala, Pedro Arias-Sanchez, Snigdha Karnakoti, Kelly M. Pennington, Elitza S. Theel, Cecilia S. Lindestam Arlehamn, Tobias Peikert, Patricio Escalante

Clinical prediction of nontuberculous mycobacteria lung disease (NTM-LD) progression remains challenging. We aimed to evaluate antigen-specific immunoprofiling utilizing flow cytometry (FC) of activation-induced markers (AIM) and IFN-γ enzyme-linked immune absorbent spot assay (ELISpot) accurately identifies patients with NTM-LD, and differentiate those with progressive from nonprogressive NTM-LD. A Prospective, single-center, and laboratory technician-blinded pilot study was conducted to evaluate the FC and ELISpot based immunoprofiling in patients with NTM-LD (n = 18) and controls (n = 22). Among 18 NTM-LD patients, 10 NTM-LD patients were classified into nonprogressive, and 8 as progressive NTM-LD based on clinical and radiological features. Peripheral blood mononuclear cells were collected from patients with NTM-LD and control subjects with negative QuantiFERON results. After stimulation with purified protein derivative (PPD), mycobacteria-specific peptide pools (MTB300, RD1-peptides), and control antigens, we performed IFN-γ ELISpot and FC AIM assays to access their diagnostic accuracies by receiver operating curve (ROC) analysis across study groups. Patients with NTM-LD had significantly higher percentage of CD4⁺/CD8⁺ T-cells co-expressing CD25⁺CD134⁺ in response to PPD stimulation, differentiating between NTM-LD and controls. Among patients with NTM-LD, there was a significant difference in CD25⁺CD134⁺ co-expression in MTB300-stimulated CD8⁺ T-cells (p