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☐ ☆ ✇ PLOS ONE Medicine&Health

Screening for antibacterial and cytotoxic activities of Sri Lankan marine sponges through microfractionation: Isolation of bromopyrrole alkaloids from <i>Stylissa massa</i>

by Lakmini Kosgahakumbura, Jayani Gamage, Luke P. Robertson, Taj Muhammad, Björn Hellman, Ulf Göransson, Prabath Jayasinghe, Chamari Hettiarachchi, Paco Cárdenas, Sunithi Gunasekera

Sri Lanka is a biodiversity hotspot and one of the richest geographical locations of marine sponges in the Indian ocean. However, the most extensive taxonomical study on Sri Lankan sponge biodiversity dates back ~100 years and only a limited number of studies have been conducted on sponge natural products. In the current study, 35 marine sponge specimens (collected from 16 sponge habitats around Sri Lanka) were identified, microfractionated and evaluated for antibacterial and anticancer assays. In total, 30 species were characterized, of which 19 species gave extracts with antibacterial and/or cytotoxic activities. Microfractionated organic extract of Aciculites orientalis gave the most potent antibacterial activity against Staphylococcus aureus and strongest lymphoma cell toxicity was exhibited by the organic extract of Acanthella sp. Guided by the molecular ion peaks in the bioactive fractions, large-scale extraction of Stylissa massa led to the isolation of three bromopyrrole alkaloids, sceptrin, hymenin and manzacidin A/C. Of these, sceptrin exhibited broad spectrum antibacterial activity against both Escherichia coli and S. aureus (MIC of 62.5 μM against both species). Based on natural product literature, seven promising species were identified as understudied. Their further exploration may lead to the discovery of structurally novel compounds.
☐ ☆ ✇ BMJ Open

Experimental studies testing interventions to promote cultural safety, interculturality or antiracism in healthcare: protocol for a systematic review

Por: Sarmiento · I. · Rojas-Cardenas · A. · Zuluaga · G. · Belaid · L. · Cockcroft · A. · Andersson · N. — Enero 3rd 2024 at 17:10
Introduction

Cultural safety, interculturality and antiracism are crucial concepts in addressing health disparities of minority and diverse groups. Measuring them is challenging, however, due to overlapping meanings and their highly contextual nature. Community engagement is essential for evaluating these concepts, yet the methods for social inclusion and protocols for participation remain unclear. This review identifies experimental studies that measure changes resulting from culturally safe, intercultural or antiracist healthcare. The review will describe outcomes and additional factors addressed in these studies.

Methods and analysis

The study focuses on epidemiological experiments with counterfactual comparisons and explicit interventions involving culturally safe, intercultural or antiracist healthcare. The search strategy covers PubMed, CINAHL, Scopus, Web of Science, ProQuest, LILACS and WHO IRIS databases. We will use critical appraisal tools from the Joanna Briggs Institute to assess the quality of randomised and non-randomised experimental studies. Two researchers will screen references, select studies and extract data to summarise the main characteristics of the studies, their approach to the three concepts under study and the reported effect measures. We will use fuzzy cognitive mapping models based on the causal relationships reported in the literature. We will consider the strength of the relationships depicted in the maps as a function of the effect measure reported in the study. Measures of centrality will identify factors with higher contributions to the outcomes of interest. Illustrative intervention modelling will use what-if scenarios based on the maps.

Ethics and dissemination

This review of published literature does not require ethical approval. We will publish the results in a peer-reviewed journal and present them at conferences. The maps emerging from the process will serve as evidence-based models to facilitate discussions with Indigenous communities to further the dialogue on the contributing factors and assessment of cultural safety, interculturality and antiracism.

PROSPERO registration number

CRD42023418459.

☐ ☆ ✇ BMJ Open

Mapping Oral health and Local Area Resources (MOLAR): protocol for a randomised controlled trial connecting emergency department patients with social and dental resources

Por: Simon · L. · Marsh · R. · Sanchez · L. D. · Camargo · C. · Donoff · B. · Cardenas · V. · Manning · W. · Loo · S. · Cash · R. E. · Samuels-Kalow · M. E. — Diciembre 10th 2023 at 15:59
Introduction

There are substantial inequities in oral health access and outcomes in the USA, including by income and racial and ethnic identity. People with adverse social determinants of health (aSDoH), such as housing or food insecurity, are also more likely to have unmet dental needs. Many patients with dental problems present to the emergency department (ED), where minimal dental care or referral is usually available. Nonetheless, the ED represents an important point of contact to facilitate screening and referral for unmet oral health needs and aSDoH, particularly for patients who may not otherwise have access to care.

Methods and analysis

Mapping Oral health and Local Area Resources is a randomised controlled trial enrolling 2049 adult and paediatric ED patients with unmet oral health needs into one of three trial arms: (a) a standard handout of nearby dental and aSDoH resources; (b) a geographically matched listing of aSDoH resources and a search link for identification of geographically matched dental resources; or (c) geographically matched resources along with personalised care navigation. Follow-up at 3, 6, 9 and 12 months will evaluate oral health-related quality of life, linkage to resources and dental treatment, ED visits for dental problems and the association between linkage and neighbourhood resource density.

Ethics and dissemination

All sites share a single human subjects review board protocol which has been fully approved by the Mass General Brigham Human Subjects Review Board. Informed consent will be obtained from all adults and adult caregivers, and assent will be obtained from age-appropriate child participants. Results will demonstrate the impact of addressing aSDoH on oral health access and the efficacy of various forms of resource navigation compared with enhanced standard care. Our findings will facilitate sustainable, scalable interventions to identify and address aSDoH in the ED to improve oral health and reduce oral health inequities.

Trial registration number

NCT05688982.

☐ ☆ ✇ Archivos de la Memoria

Vacaciones para trabajar duro. Experiencia personal de una cirujana voluntaria

Por: Sonia Sanchiz Cárdenas — Enero 27th 2022 at 09:39

En este relato biográfico contamos la experiencia de una cirujana como voluntaria en varios proyectos de cirugía en países con bajos recursos. El objetivo es conocer cómo funcionan estos proyectos, la labor que llevan a cabo y la implicación física y psicológica que supone. Se realiza mediante un abordaje cualitativo de una entrevista semiestructurada para analizar diferentes aspectos. El informante aporta datos sobre la organización del proyecto y el trabajo que realizan, su visión sobre estos proyectos y sus vivencias. Cuenta con naturalidad y simpatía sus experiencias en Bolivia y Ghana, resalta su humildad a la hora de contar su participación y las críticas que realiza.

☐ ☆ ✇ Evidentia

Plan de cuidados desde clínica de heridas para el salvamento del pie diabético, un caso de éxito

Este caso clínico presenta la situación de un paciente de 66 años insulino requirente, quien se negó a realizar amputación digital transmetatarsiana en el pie derecho como única opción de tratamiento para el pie diabético, el paciente fue atendido en clínica de heridas donde se elaboró el plan de cuidados utilizando el modelo de enfermería de Virginia Henderson, las taxonomías de la North American Nursing Diagnosis Association (Nanda), Nursing Outcomes Classification (Noc) y Nursing Interventions Classification (Nic). Se establecieron como diagnósticos enfermeros: deterioro de la integridad tisular, perfusión tisular periférica ineficaz y gestión ineficaz de la salud. El desbridamiento mecánico del tejido necrótico bajo la aplicación de procesos de asepsia, el compromiso del grupo multidiscipli-nario de la salud y la educación al paciente, permitieron el salvamento de 2 falanges del dedo y el retorno a la vida normal del paciente en un periodo de 7 meses.

☐ ☆ ✇ Evidentia

Expediente Electrónico vs Expediente Físico para mejorar la calidad del proceso de atención en pacientes hospitalizados

Objetivo principal: Comparar la eficacia del uso del Expediente Clínico Electrónico (ECE) Vs Expediente Clínico Físico (ECF) en la mejora la calidad en atención de pacientes hospitalizados. Metodología: Se consideraron estudios relacionados con uso del ECE, utilizando los descriptores DeCS y MeSH, operadores booleanos AND, NOT y OR. Criterios inclusión: artículos de revisión con cinco años de publicación; criterios exclusión: pacientes ambulatorios o de consulta externa. Resultados principales: La evidencia establece ventajas del uso ECE como la facilidad de uso, accesibilidad, lectura, calidad en la documentación, control de información sobre los cuidados de los pacientes, cumplimiento normativo y toma de decisiones, lo cual reduce el riesgo de errores médicos; sin embargo, algunas desventajas es el empleo de hardware, software, redes; requiere soporte técnico las 24 horas al día. Conclusión principal: el empleo del ECE establece eficacia en la atención al paciente hospitalizado mediante la minimización de tiempos en registros y el aumento en la seguridad de la información.

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